Journal: Cell Reports
Article Title: Monoclonal antibodies protect aged rhesus macaques from SARS-CoV-2-induced immune activation and neuroinflammation
doi: 10.1016/j.celrep.2021.109942
Figure Lengend Snippet: mAbs limit SARS-CoV-2-induced systemic immune activation (A and B) Gating strategy and frequencies of (A) neutrophils (CD3/20 − HLA-DR − CD66 + ) at days −3 and 0 (pre) versus days 3 and 7 (post) and (B) Ki-67 + PD-1 + T fh cells (CD95 + CXCR5 + ) in whole blood (n = 4 in each group). (C) Gating strategy and frequency of CD8 + CD69 + T cells expressing Ki-67, PD-1, or granzyme B (GzmB) in liver and spleen 7 days pi. (D) Heatmap displaying log2 normalized protein expression (NPX) values from plasma inflammatory proteins (top) and kinetics of NPX for plasma CXCL9, CXCL10, and CXCL11 (bottom). n = 4 for each experimental group/sample time point. (E) Serum levels of anti-nuclear antibodies (ANAs) and anti-phospholipid antibodies (aPLs) in aged and young animals on days 0 and 7 pi (young, n = 4; aged, n = 8). (F and G) Comparison of sgRNA and vRNA in (F) NSs and (G) BAL in aged and young animals on days 3 and 7 pi (young, n = 4; aged, n = 4). Data points represent individual animals, with bars indicating medians. Gray bars indicate limit of detection. Flow data and NPX data represent a single technical replicate. ∗ p < 0.05 by two-tailed (A and B) or one-tailed Mann-Whitney U test (C–G).
Article Snippet: Mouse anti-human CD66 (Clone TET2) , Miltenyi Biotec , Cat#130-118-539; RRID: AB_2733398.
Techniques: Activation Assay, Expressing, Clinical Proteomics, Comparison, Two Tailed Test, One-tailed Test, MANN-WHITNEY